Determination by Converting Menadione Sodium Bisulphite to Menadione in Pharmaceutical Preparation by Two Derivative Spectrophotometric Methods
نویسندگان
چکیده
Firstand second-order derivative spectrophotometric methods for the quantitative determination by converting menadione sodium bisulphite (MSB) to menadione (M ) with sodium carbonate were developed and validated in pharmaceutical preparation. The quantitative determination of MSB was carried out by using wavelengths 350 nm (for first-order; 1D) and 355 nm (for second-order; 2D) with the linearity ranges 0.5-40 μg/mL M (i.e.0.82-65.6 μg/mL MSB). The percentages of conversion MSB to M obtained with methods were more than 97.1 %. The proposed derivative spectrophotometric methods easily applied to commercial ampoule containing MSB and were statistically compared.
منابع مشابه
GC-FID and HPLC-DAD Methods for the Determination of Menadione Sodium Bisulphite Directly and by Converting Menadione Sodium Bisulphite to Menadione in Pharmaceutical Preparation
GC-FID and HPLC-DAD were developed for determination of menadione (MN) and menadione sodium bisulphite (MSB). By means of each method, quantitative analysis of MSB in commercial pharmaceutical was performed in both direct analysis of MSB and analysis of MN by converting MSB to MN with sodium carbonate. GC-FID method was carried out on the HP-5 capillary column using nitrogen gas. HPLC-DAD metho...
متن کاملGC-FID and HPLC-DAD Methods for the Determination of Menadione Sodium Bisulphite Directly and by Converting Menadione Sodium Bisulphite to Menadione in Pharmaceutical Preparation
GC-FID and HPLC-DAD were developed for determination of menadione (MN) and menadione sodium bisulphite (MSB). By means of each method, quantitative analysis of MSB in commercial pharmaceutical was performed in both direct analysis of MSB and analysis of MN by converting MSB to MN with sodium carbonate. GC-FID method was carried out on the HP-5 capillary column using nitrogen gas. HPLC-DAD metho...
متن کاملGC-FID and HPLC-DAD Methods for the Determination of Menadione Sodium Bisulphite Directly and by Converting Menadione Sodium Bisulphite to Menadione in Pharmaceutical Preparation
was performed in both direct analysis of MSB and analysis of MN by converting MSB to MN with sodium carbonate. GC-FID method was carried out on the HP-5 capillary column GC-FID and HPLC-DAD methods were developed for determination of menadione (MN) and menadione sodium bisulphite (MSB). By means of each method, quantitative analysis of MSB in commercial pharmaceutical using nitrogen gas. HPLC-D...
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